Our dedicated GEMSTONE researchers are fully immersed in the fascinating world of confocal microscopy, a cutting-edge technique that holds tremendous significance for our research targets at Acibadem University (ACU).
Confocal microscopy offers an innovative solution by effectively eliminating out-of-focus light, resulting in high-resolution imaging even in thick tissues. This powerful method allows us to delve deeper into the intricacies of neurobiology and unravel the mysteries of brain function.
To ensure we achieve the highest level of quality outputs, our researchers recently embarked on an inspiring journey to Lund University (ULUND). Collaborating with experts in the field, we are determined to engage in the same level of qualified outputs and expand our scientific horizons.
One of the techniques we are employing is indirect (two-stage) immunofluorescence, a game-changer in imaging. By leveraging a combination of primary and secondary antibodies, we can visualize highly specific targets with exceptional precision.
In brain tissue, we first treat the antigenic determinants of interest with a primary antibody and then apply a secondary antibody, which is directed to the host primary. This secondary antibody is conjugated to a synthetic or natural fluorophore, enabling us to capture remarkable images and gain deeper insights into the complex neural network.